BIOINFORMATICS
Nanotechnology for Cancer
JOHNS HOPKINS UNIVERSITY
NEW DRUG DEVELOPMENT
At Johns Hopkins University, I worked towards novel chemotherapeutic drug development for breast and colon cancer treatment. The drug target was a helicase gene referred to as DDX3. DDX3 is overexpressed in cancer types, and it is recognized for causing a low survival. To inhibit the expression of DDX3, the laboratory developed a treatment line known as RK-33. RK33 inhibits resulted in the activation of the cell death pathway, abrogation of non-homologous end-joining activity, and inhibition of the Wnt-signaling pathway. RK-33’s is undergoing pre-clinical trials and its effects are being tested on both invitro mice models and radiological implications of the same.
DDX3 BIOCHEMICAL GENE EXPRESSION PATHWAY.
I tested newly formulated drug on cancer cell lines such as MDA-MB-231 and many others, by performing multiple cell death assays such as MTS Assays (MTT assay that uses tetrazolium dye for cell vitality assay). I also participated in developing Experimental designs on animal models for tumor implantation in mice. I run the following experiments in mice models by drug injection and mice exposed to different radiation levels to see the individual effect of RK-33 and the combined effect of RK-33 and radiation.
I analyzed SNP Data using Bio-python packages, managed the wet lab, and performed Bradford Assay, Western Blot, for the various cancer cell lines.
Attended lectures and interacted with Nobel laureates. Managed regular lab workings and restocked and maintained the Laminar flow hoods and cell incubators.
JOHNS HOPKINS UNIVERSITY: SCHOOL OF MEDICINES
IS ONE OF THE MOST BEAUTIFUL AND CULTURALLY
ENRICHED CAMPUSES I HAVE EVER VISITED.
JOHNS HOPKINS UNIVERSITY PROVIDED ME WITHAN EXTREMELY SUPPORTIVE AND DIVERSE GROUP OF INTELLECTS AT ONE PLACE THAT THEY NEVER FAILED TO AMAZE ME.
UNIVERSITY OF PITTSBURGH SCHOOL OF PHARMACEUTICAL SCIENCES
Bone Regeneration Scaffolds
Built Polysaccharides hydrogel beaded scaffolds and optimized the building conditions for the same. The non- toxic scaffolds were then implanted in mice skull model for drug delivery to test the drug’s bone regeneration ability the scaffold itself when a circular shaft of more than 2mm drilled into a mice skull.
Performed decalcification and storage parts of mice skull. Then created wax blocks using a HistoEmbedder and evaluated and wrote a protocol on the Histo-Embedder for regular lab use.
Monitored the impact of oxaloacetate ions, on breast cancer tumor microenvironment to test the hypothesis of whether breast cancer is primarily metastasis to bone cancer.
Maintained laboratory records and stored sacrificed mice bones.
AUTOMATIC SYRINGE PUMP
HISTO EMBEDDER